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Primer Premier 5.0 Description: Primer Premier is a comprehensive primer design application. Primer Premier 5.0 Features: PCR primer design- design primer pairs for PCR and multiplex experiments. Cross species- Amplify sequences from multiple species. Pathogenic detection- Locate oligos in highly conserved regions. Allele specific- Exclusively amplify a single member of a set of related sequences.

Degenerate- Start with an amino acid sequence, reverse translate and design oligos in regions of low degeneracy automatically. Nested/multiplex- Checking homology among multiple candidate oligos. Restriction enzyme and motif analysis- Select from the database of over 800 enzymes and 200 common motifs. Clustal W alignments- Load the aligned sequences after performing ClustalW alignment using any resource, including EBI. Multiple sequence alignment- Use the popular Clustal W algorithm to align sequences from your desktop (available in Windows version only). Algorithm- Calculates Tm using Breslauer values. Long PCR- Handles sequences up to 50 KB.

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